STUDIES ON l-GLUTAMIC ACID DECARBOXYLASE FROM ESCHERICHIA COLI
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چکیده
منابع مشابه
Arginine Decarboxylase from Escherichia coli
A total of three pyridoxyl-peptides were isolated from the inducible argmine decarboxylase of Escherichia coli B following reduction with NaBH4 and proteolysis with trypsin or chymotrypsin. Sodium borohydride reduces the Schiff base formed between pyridoxal-5’-P and the e-amino group of a lysyl residue of the protein. The sequence analysis of the three peptides is consistent with a unique pyrid...
متن کاملBiosynthetic Arginine Decarboxylase from Escherichia coli
Biosynthetic arginine decarboxylase of Escherichia coli has been purified 1,500-fold. The purified enzyme showed two bands on disc gel electrophoresis, both carrying out the decarboxylation of L-arginine. Electrophoresis in the presence of sodium dodecyl sulfate and sedimentation equilibrium ultracentrifugation was consistent with the enzyme being a tetramer of approximately 296,000 molecular w...
متن کاملINVESTIGATION ON ANTI-GLUTAMIC ACID DECARBOXYLASE ANTIBODIES IN TYPE I DIABE TES MELLITUS
Antibodies directed against the enzyme glutamic acid decarboxylase (GAD) are believed to be the main cause of destruction of pancreatic islet cells in type I (insulin dependent) diabetes mellitus. The enzyme was found both in the brain and pancreatic beta cells. Although similarities in identity of GAD in human and rat brain have been demonstrated, little is known about the interaction betw...
متن کاملMetabolism of glutamic acid in a mutant of Escherichia coli.
Vender, Joyce (Indiana University, Bloomington), Kunthala Jayaraman, and H. V. Rickenberg. Metabolism of glutamic acid in a mutant of Escherichia coli. J. Bacteriol. 90:1304-1307. 1965.-A mutant strain of Escherichia coli W1485 was selected for its ability to utilize glutamic acid as the sole source of carbon. Growth of the mutant on glutamic acid led to the repression of glutamic acid dehydrog...
متن کاملDeoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r.
An increase in the rate of synthesis of ara-specific messenger ribonucleic acid as measured by deoxyribonucleic acid-ribonucleic acid hybridization has been detected in the induced wild-type (ara(+)) strain of Escherichia coli B/r as compared with the uninduced control, thus providing evidence that regulation of the positively controlled l-arabinose operon is at the level of transcription.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1954
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71312-4